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Fig. 4 | BMC Plant Biology

Fig. 4

From: Evolution, gene expression, and protein‒protein interaction analyses identify candidate CBL-CIPK signalling networks implicated in stress responses to cold and bacterial infection in citrus

Fig. 4

Expression patterns of eight CsCBLs and 17 CsCIPKs in leaves of healthy sweet orange trees and in leaves of those infected with CLas as determined by qRT‒PCR. Healthy and CLas-infected leaves from 15-year-old ‘Yuanfeng’ navel orange trees were collected in the same orchard in Wugang County of Hunan Province. Three biological replicates were used for measuring gene expression, and the experiment was repeated once with similar results. The relative expression levels of candidate genes were calculated using the 2−∆∆CT method. The CsEF1α gene was used as an internal reference for the nominalization of the transcript levels of the genes tested. Error bars indicate inferential statistics according to the SE. *indicates statistical significance (*p < 0.05; **p < 0.01; ***p < 0.001; Student’s t test) when compared to the expression level in leaves of healthy sweet orange trees

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BMC Plant Biology

ISSN: 1471-2229

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