Fig. 1

Chromosomal locations of CsCBLs (A) and CsCIPKs (C) and the phylogenetic trees based on the predicted CuCBL and CuCIPK protein sequences along with their sequence alignment in the conserved domains (B and D). Note that only chromosomes with CsCBL and CsCIPK genes are shown in the drawing. The phylogenetic trees for CuCBLs and CuCIPKs of ‘Guijing2501’ satsuma mandarin were generated using the maximum likelihood method. Four EF-hand domains, PFPF motifs and the lengths of the linker amino acid sequences for CuCBL proteins are shown in B. The ATP-binding site and activation loop in the N-terminal catalytic kinase domain and the NAF/FISL and PPI motifs in the C-terminal regulatory domain for CuCIPK proteins are shown in D