Fig. 8

Effects of silencing of Gh_SCPL42 on Zhongzhimian 2 susceptibility to V991. Two weeks after infiltration, seedlings were inoculated with V991. A Seven day-old cotton plants were infiltrated with Agrobacterium carrying TRV::GhCLA. The photographs were taken at 2 weeks after infiltration. B qRT-PCR for detection of silencing efficiency. C Representative seedlings of control (CK) and silenced plants after inoculation with V991 at 21 days post-inoculation (dpi). D Responses of control (CK) (TRV::00) and silenced (TRV::Gh_SCPL42) plants to the V991 at 21 dpi. Disease severity index (DSI) in CK and silenced plants each with 20 experimental replicates. The DSI were measured at 21 dpi. Error bars represent the standard deviation of 20 biological replicates; asterisks indicate statistically significant differences, as determined by t-test (*P < 0.05; **P < 0.01, ***P < 0.01). E qRT-PCR of genes related to SA, JA, ET and lignin pathway