Fig. 6

Effects of PhUGT78A22 silencing on P. ‘He Xie’ bud scales. A Silencing of PhUGT78A22 changed bud scale color. Representative images of TRV control and TRV::PhUGT78A22 treated bud scales were taken 3 d after infiltration. Scale bar, 2 mm. B RT-qPCR analysis of PhUGT78A22 in TRV control and PhUGT78A22-silenced P. ‘He Xie’ bud scales. One biological sample consisted of a mixture of at least 6 bud scales. The mean values ± SD from three biological replicates (n = 3) are shown. Asterisks indicate statistically significant differences (two-sided Student’s t-test; *, P < 0.05). C CIELAB color space analysis of TRV control and PhUGT78A22-silenced P. ‘He Xie’ bud scales. The mean values ± SD from three biological replicates (n = 6) are shown. Asterisks indicate statistically significant differences (two-sided Student’s t-test; **, P < 0.01). D Silencing of PhUGT78A22 altered anthocyanin accumulation in P. ‘He Xie’ bud scales. Anthocyanin accumulation was determined using UPLC-DAD analysis in TRV control and PhUGT78A22-silenced bud scales. One biological sample consisted of a mixture of at least 6 bud scales. The mean values ± SD from three biological replicates (n = 3) are shown. Asterisks indicate statistically significant differences (two-sided Student’s t-test; **, P < 0.01)