Fig. 7

Effects of GhACS2 and GhACS6 activities on SA biosynthetic and signaling events. A qRT-PCR analysis of the expression levels of SA biosynthetic and signaling genes (EDS1, PAD4, NPR1, PR1 and PR5) in GhACS2-OE and GhACS6-OE plants. GhUBQ7 was used as the internal control to normalize all the data. Experiments were repeated three times with similar results. Values are means ± SDs (Student’s t-test; *P < 0.05; **P < 0.01). B HPLC analysis of SA levels in roots of YZ1, GhACS2-OE and GhACS6-OE plants at 16 days after Vd991 inoculation. Experiments were repeated three times with similar results. Values are means ± SDs (Student’s t-test; *P < 0.05; **P < 0.01)