Fig. 5
From: Suppression of GhGLU19 encoding β-1,3-glucanase promotes seed germination in cotton
Glycolysis and pyruvate metabolism pathway was changed in early germination of transgenic plants. A-B KEGG pathway analysis of DEPs (A) and DEGs (B) from GhGLU19-overexpressing line OE4 compared to W0 indicated that glycolysis and pyruvate metabolism were highly enriched. C Genes related to glycolysis and pyruvate metabolism were highly expressed in OE4. These genes were selected from KEGG map (ko00620 and ko00010) [21]. D Expression clarification of glycolysis and pyruvate metabolism related genes in transgenic and W0 seeds by qRT-PCR. Genes related to glycolysis and pyruvate metabolism were involved in PFK, ALDO, PK and PDH. Their expression levels were significantly evaluated in 8 h imbibed seeds of GhGLU19-overexpressing transgenic lines compared to W0. Relative expression was calculated using Histone3 (AF024716) as an internal reference. Three independent biological replicates were analyzed with three technical repeats. Vertical bars represent means ± SD. * p < 0.05. ** p < 0.01