Fig. 3
From: Suppression of GhGLU19 encoding β-1,3-glucanase promotes seed germination in cotton
Seed germination and water absorption percentages in GhGLU19-overexpressing and GhGLU19-suppressing lines. A Photographic illustration of seed germination curve at 24, 36, 48, 72 and 96 h after imbibition. Scale bar, 3 cm. B Line chart of seed germination percentages in transgenic lines and W0 after 24, 36, 48, 60, 72, 84 and 96 h at 25 °C growth chamber. Germination percentage as the means ± SD with three biological replicates. C-D Water uptake time course of transgenic and wild-type seeds. Seeds from W0, GhGLU19-suppressing lines AS19 and AS105, GhGLU19-overexpressing lines OE4 and OE110, were sown in dishes at 25 °C, respectively. Percentage of water uptake was scaled by 2 h over one day. After 24 h, water uptake percentage was calculated each 12 h interval. Three independent biological replicates were analyzed. Water uptake percentage as the means ± SD, student’s t-test, * p < 0.05, ** p < 0.01