Fig. 1

Phylogenetic analysis, conserved domain identification, and miR319-binding site recognition of CsTCPs. A Phylogenetic tree of Citrus sinensis, Arabidopsis thaliana, and Solanum lycopersicum TCP proteins. A total of seventy-four TCP protein sequences were analyzed by the Muscle method, and the neighbor-joining tree was constructed with MEGA X and iTOL v.6. Purple, orange, and blue regions represent PCF subclade, CIN subclade, and CYC/TB1 subclade, respectively. The putative CsTCPs are shown in red. The bootstrap test was implemented with 1000 iterations. Size of green dot represents bootstrap value. B Multiple sequence alignment of the TCP domain in Citrus sinensis and Arabidopsis thaliana TCP members. The alignment was performed by the Muscle method with MEGA X and was visualized using Jalview v.2.11.1.4. The conserved regions (BASIC, HELIX I, LOOP, and HELIX II) of TCP domain are indicated at the top. The conserved amino acids are in black. C Multiple sequence alignment of the R domain in TCP members. The conserved amino acids are in black. D Analysis of the putative target sites for miR319 in CsTCPs and AtTCPs. The alignment was conducted using psRNATarget