Fig. 7
From: MdNup62 interactions with MdHSFs involved in flowering and heat-stress tolerance in apple
MdNup62 interacts with MdHSFs. a Interactions between MdNup62508−613 and MdHSFs (MdHSFA9a/b and MdHSFA1a/b/d/e) in Y2H assays. The MdNup62508−613 truncated sequence was cloned into pGBKT7, whereas MdHSFAs (MdHSFA9a/b and MdHSFA1Da/b/d/e) were cloned independently into the pGADT7 vector. Empty pGADT7 plus MdNup62.508−613-pGBKT7 was used as the control. b Interactions between MdNup62 and MdHSFA9b in the pull-down assay. Western blotting with a GST antibody revealed that MdNup62-HIS was pulled down by MdHSFA9b-GST. c–e Interactions between MdNup62 and both MdHSFA9b and MdHSFA1d in a luciferase (LUC) complementation experiment. Empty NLUC and empty CLUC, MdNup62-NLUC plus empty CLUC, empty NLUC plus MdHSFA9b, and MdHSFA1d-CLUC were used as controls