Fig. 2

Structure of KoWRKY40 protein and comparison with its template AtWRKY1. The 3D structural model of KoWRKY40 was generated based on its template AtWRKY1 (SMTL id: 2ayd.1.A) through homology modeling SWISS-MODEL. The sequence identity between them is 51.35%. A Cartoon representation of KoWRKY40 protein. The KoWRKY40 is composed of one α-helix (purple curl) and five β-strands (green ribbons), which are numbered from the N-terminus. The claret regions of the cartoon structure represented zinc-binding sites. The zinc ion and zinc coordination residues are represented by purple spheres and rods (gray for C, red for O, blue for N, orange for S), respectively. B The charge distribution on the surface of KoWRKY40 structure. Negative charges are marked by red, and positive charges are indicated by blue. C 3D-superimposition structure of KoWRKY40 and the best representative structure of 2ayd.1.A. The macromolecular structures are shown by traces, with the KoWRKY40 colored in khaki and 2ayd.1.A in cyan. The zinc ions are shown by purple spheres in both KoWRKY40 and 2ayd.1.A. Succinic acid are represented by sticks in 2ayd.1.A. D Model-template alignment of KoWRKY40 and 2ayd.1.A. The β-strands were colored green, α-helix was colored purple. The claret residues represented zinc-binding sites, marked by yellow background in Fig. 1, which interacted with zinc-coordinating residues