Fig. 6

Differential expression of DcRAD51D, OSDLa and DcPS1 in the wild type and corresponding DcPS1 RNAi line and OSDLa RNAi line. For quantitative expression analysis, RT-qPCR was performed on RNA harvested from young leaf. (A) Transcript levels of DcRAD51D, OSDL1a and DcPS1 were quantified in the wild type and corresponding DcPS1 RNAi line. (B-E) Transcript levels of DcRAD51D, OSDL1a and DcPS1 were quantified in the wild type and corresponding OSDLa RNAi plant B1, C1, C2, G3. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used to normalize initial cDNA concentrations. Comparative statistics of the means were checked using a oneway ANOVA test (SPSS 18.0)