Fig. 9

Effect of Os02g0817500 knockout on the seed vigor of rice ‘Nipponbare’. A Target sites in the wild type (WT) are marked in red box. Inserted nucleotide is indicated with red uppercase letter and red dashes represent the deleted bases. The original data can be viewed from Additional file 4: Fig. S5. B Analysis of the Os02g0817500 protein sequence in the wild-type (WT) and Os02g0817500-knockout lines. C Photographs of germinated seeds before and after artificial aging of Os02g0817500-knockout lines and ‘Nipponbare’ (WT) after 5 days after germination. (D, E) Comparison of germination rate (D) and simple vigor index (E) between Os02g0817500-knockout lines and ‘Nipponbare’. Bar = 1 cm. Each column presents the means ± standard deviations of three biological replicates. *P < 0.05, **P < 0.01 and ***P < 0.001 compared with the WT by Student’s t-test