Fig. 1

Level of StGI.04 repression in aGI lines compared to the non-transformed control ‘Désirée’ (DES). RNA was isolated from (a) middle leaves of in vitro plants; 3 leaves/line harvested from 3 plants, (b) source leaves of 8-week-old plants grown in pots in a greenhouse; 9 leaf discs of 1 cm in diameter/line harvested from 9 plants, (c) mature tubers harvested at the end of the vegetation period; 3 sets/line, 3 tubers/set composed from the largest tubers of each line distributed into approximately equal groups. Y-axis shows mean relative expression values of StGI.04 gene compared to the mean expression values of ACTIN. The means were calculated from three technical replicates in case of (a) and (b) and from 3 biological replicates in case of (c). The standard deviations are indicated by the error bars. Significant differences between the aGI lines and DES were detected by Student’s t-test and labelled by * (p < 0.05) and ** (p < 0.01)