Combined morpho-physiological, ionomic and transcriptomic analyses reveal adaptive responses of allohexaploid wheat (Triticum aestivum L.) to iron deficiency

Table 1 Statistics of wheat transcriptome sequencing data under low Fe stress

Sample	Raw reads	Clean reads	Mapped Reads	Error rate(%)	Q20(%)	Q30(%)	GC content(%)
HIR1	103,367,072	102,485,512	93,941,511 (91.66%)	0.0244	98.24	94.79	55.13
HIR2	92,962,154	91,950,192	82,671,080 (89.91%)	0.0246	98.16	94.60	54.20
HIR3	93,650,802	92,663,246	83,454,929 (90.06%)	0.0246	98.17	94.63	54.67
HIS1	81,831,040	81,022,372	75,929,551 (93.71%)	0.0249	98.03	94.26	55.55
HIS2	97,354,592	96,467,486	90,270,260 (93.58%)	0.0244	98.21	94.75	56.63
HIS3	109,751,026	108,750,122	102,330,323 (94.10%)	0.0244	98.24	94.80	55.66
LIR1	88,487,912	87,710,632	81,340,207 (92.74%)	0.0247	98.12	94.44	55.03
LIR2	84,443,470	83,702,758	76,906,608 (91.88%)	0.0248	98.08	94.38	54.53
LIR3	108,537,716	107,600,262	100,077,545 (93.01%)	0.0245	98.19	94.64	55.20
LIS1	85,080,798	84,380,564	79,656,284 (94.40%)	0.0241	98.33	95.06	57.10
LIS2	106,018,904	105,038,702	98,685,159 (93.95%)	0.0245	98.19	94.67	55.62
LIS3	93,658,786	92,779,306	87,098,185 (93.88%)	0.0247	98.10	94.43	55.92

HIR Hight Iron Root, HIS Hight Iron Shoot, LIR Low Iron Root, LIS Low Iron Shoot. 1, 2, and 3 respectively represent different biological repetitions. Error rate (%) is the average error rate of sequencing bases corresponding to the quality control data. Q20% is the proportion of the nucleotide quality value larger than 20; Q30% is the proportion of the nucleotide quality value larger than 30; GC content (%) is proportion of guanidine and cytosine nucleotides among the total nucleotides

ISSN: 1471-2229