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Fig. 7 | BMC Plant Biology

Fig. 7

From: Genome-wide identification and expression analysis of the SWEET gene family in daylily (Hemerocallis fulva) and functional analysis of HfSWEET17 in response to cold stress

Fig. 7

Subcellular localization of HfSWEET17 fusion protein. The 35:HfSWEET17-YFP was generated by the insertion of the open reading frame (ORF) of HfSWEET17 into the pC131-YFP vector framework, and transformed into GV3101. Organelle markers, including endoplasmic reticulum (ER) marker HDEL-mCherry, and nuclear localization sequence (NLS) marker pBin-NLS-mCherry, were used in co-transformation experiments with the 35S:HfSWEET17-GFP to determine its subcellular localization. Confocal laser microscopy scanning was carried out 48 h after dark culture. Representative images are shown. Leaves transformed with the 35S:YFP vector alone were used as a control

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BMC Plant Biology

ISSN: 1471-2229

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