Fig. 2

Expression of CrWUL in the sporophyte generation of C. richardii. (a-d) Stages of wild-type sporophytes used for expression assays; sporophyte with one leaf (p2) (a), six leaves (p7) (b), 13 expanded leaves (p13) (c), and mature sporophyte with sporophyll (d). (e)Expression of CrWUL in sporophyte tissues measured by RT-qPCR (mean ± SEM;**, P ≤ 0.01; ****, P ≤ 0.0001; N = 3): p2 (the whole plant), p7 (the whole plant), L (oldest fully expanded leaf), R (root), S (shoot) from a liquid grown p13 plant; v.petiole ( vegetative petiole), v.blade (vegetative leaf blade), s.petiole (sporophyll petiole), s.blade (sporophyll blade) from a mature sporophyte shown in (d). (f-k) In situ hybridization of sectioned sporophyte tissues; SAM (f), RAM (g), young petiole (h); cross section of vegetative petiole (i), emerging leaf blade (j), and root vascular tissues (k). White arrowhead points to leaf primordium. Purple arrowheads denote shoot (f) and root (g) apical cells, respectively. Yellow arrowheads indicate phloem tissues. Scale bars = 1 mm (a), 4 mm (b), 25 mm (c), 46 mm (d), 0.05 mm (f-k)