Fig. 1

Expression of CrWUL in the gametophyte generation of C. richardii. (a-c) Representative wild-type hermaphrodite gametophytes at 7-, 9-, and 13-days post plating. (d) Expression of CrWUL during gametophyte development measured by RT-qPCR (mean ± SEM; *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; N = 3). (e–g) Whole mount in situ hybridization of 7-day (g), 9-day (h), and 13-day (i) old hermaphroditic gametophytes. Black arrowhead in (a-c) indicates notch meristem. Scale Bars = 0.05 mm