Fig. 3

Summary of the transcriptome of sugarcane in ratoon chlorosis leaves and planted plantlet green leaves. Transcriptome de novo assembly completeness analysis using BUSCO alignment (A). Complete orthologues include a single copy (blue) and duplicated copies (green). Incomplete orthologues are fragmented (yellow). The missing (red) copies are not found in the BUSCO database. The Venn diagram of aligned and annotated assembly using multi databases (B). The distribution of species annotation unigenes (C). The volcano plot of expression unigenes (D). The up-and down-regulated genes are represented as red and yellow dots, while the light blue dots indicate the unigenes without significant changes. The unigenes with a fold change higher than 2 and a p-value lower than 0.05 are determined as DEGs