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Fig. 7 | BMC Plant Biology

Fig. 7

From: Small HSPs play an important role in crosstalk between HSF-HSP and ROS pathways in heat stress response through transcriptomic analysis in lilies (Lilium longiflorum)

Fig. 7

Decreased expression of LlHsfA2 caused sensitive phenotype of heat stress. A Thermotolerance of LlHsfA2-silenced lily lines was reduced after 24 h heat stress of 42 °C. B The detection of mRNA level of HsfA2 in LlHsfA2-silenced lily lines, control lily lines and wild type (before injection) by qRT-PCR. C The detection of mRNA level of LlHsfA2 in the leaves of LlHsfA2 silenced lily lines and control lily lines after 1d of heat stress by qRT-PCR. D The detection of mRNA level of LlHsfA2 putative down-stream genes in LlHsfA2-silenced lily lines after 1d of heat stress by qRT-PCR. The lily housekeeping gene, 18S rRNA gene, was used as an internal control for normalization in qRT-PCR and relative gene expression levels were calculated using the 2−ΔΔCT method. Data are means ± SD of three biological replicates. Different letters indicate significant differences between groups at P < 0.05 according to Duncan’s multiple range test

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BMC Plant Biology

ISSN: 1471-2229

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