Fig. 8

The PLD inhibitor affects the expression of ACC-responsive genes in N. yezoensis. RNA samples were prepared from gametophytes that were cultured in media containing 0 (Control) and 50 μM ACC (ACC) and from those containing co-applications with 0.02% 1-butanol (1-But + ACC), 0.02% t-butanol (t-But + ACC), 0.02% 1-butanol (1-But), or 0.02% t-butanol (t-But) alone for 3 d. Expression levels were determined using the Ny18SrRNA gene for normalization. NyGT14, NyVWA1, and NyVWA2 were selected as representative genes that were upregulated by ACC, whereas NySIG1, NyPsbQ, and NyAly were selected as representative genes that were downregulated by ACC genes. These results are presented as relative expression level at the given time point relative to that present in untreated gametophytes (0 d). All data are presented as mean ± SD from three independent experiments