Fig. 7

A PLD inhibitor blocks 1-aminocylopropane-1-carboxylic acid (ACC)-induced sexual reproduction. A Suppression of progression of maturity in the presence of the PLD inhibitor. Gametophytes were cultured in media containing 0 (Control) and 50 μM ACC (ACC) or a combined treatment of ACC and 0.02% 1-butanol (1-But + ACC), ACC and 0.02% t-butanol (t-But + ACC), 0.02% 1-butanol without ACC (1-But), or 0.02% t-butanol (t-But) alone. Plant maturity levels are illustrated as Fig. 6. Data were calculated in four independent experiments with four thalli for each condition (n = 16). B Alleviation of ACC-induced growth inhibition in the presence of the PLD inhibitor. Data are expressed as mean ± SD of four independent experiments with four thalli for each condition (n = 16). Asterisks indicate significant differences at P < 0.01 between the controls and treatments. C We also observed suppression of ACC-induced carpospore formation in the presence of the PLD inhibitor. The number of carpospores released from gametophytes was counted under microscope after 14d of culture. Data are expressed as means ± SD of two independent experiments with four thalli for each condition (n = 8)