Fig. 5

Effects of the PLD inhibitor 1-butanol in the presence of 1-aminocylopropane-1-carboxylic acid (ACC). Gametophytes were cultured in media containing 0 (Control) or 50 μM ACC (ACC). Co-treatments included a 10-day application of ACC with 0.02% 1-butanol (1-But + ACC), 0.02% t-butanol (t-But + ACC), 0.02% 1-butanol (1-But - ACC), and 0.02% t-butanol (t-But - ACC) alone for 10 d. We found that the thalli treated with ACC alone or by co-treatment with ACC and t-But formed many spermatangia in the upper regions and that these were often clear or discolored. Other thalli exposed to co-treatment with ACC and 1-But exhibited no discoloration in the upper regions. Scale bar = 10 mm