Fig. 5
Oxidative stress in tea plant roots upon Al stress. a, b 2,7-DCFDA staining of ROS (a) and DAB staining of H2O2 (b) in tea seedling roots in response to 0, 0.4, 2.5, and 5 mM Al2(SO4)3. The root tips were stained with 2,7-DCFDA or 3,3-diaminobezidin (DAB) in 10 mM Tris-HCl (pH 7.6) and then imaged using a fluorescence microscope. c Heatmap analysis of expression of glutaredoxin (CsGRX) genes in tea seedling roots in response to Al stress. d Analysis of expression patterns of superoxide dismutase (CsSOD) and catalase (CsCAT) genes in tea seedling roots in response to Al stress. The data are the ratio of transcriptome data at different treatment times. e Analysis of expression patterns of ascorbate peroxidase (CsAPX) and peroxidase (CsPOD) genes in tea seedling roots in response to Al stress. Data are presented as averages of calculated FPKM readings and representative fluorescence microscopy images are shown. All results were from three independent experiments