Fig. 6From: Genome-wide identification and characterization of bZIP gene family and cloning of candidate genes for anthocyanin biosynthesis in pomegranate (Punica granatum)Cloning and characteristics of PgbZIP16 and PgbZIP34. a. PCR amplification of PgbZIP16 and PgbZIP34. M: DL ladder 2000 DNA Marker. 1: PgbZIP16. 2: PgbZIP34. b. Three different colors of pomegranate flowers. c. Subcellular localization of the 35S::GFP-PgbZIP16 and 35S::GFP-PgbZIP34 fusion protein in tobacco leaves. Free GFP served as a control. A DAPI staining assay was conducted to confirm the nuclear localization. Bars = 50 μm. d. Tissue petal stage-specific expression of PgbZIP16 and PgbZIP34 in pomegranate. Different lowercase letters indicate significant differences at the 0.05 levelBack to article page