Fig. 3
From: MNB1 gene is involved in regulating the iron-deficiency stress response in Arabidopsis thaliana
The expression pattern of MNB1. A, B The expression pattern of MNB1 was induced under iron-deficient. qRT-PCR analysis of FIT and MNB1 transcript accumulation in the roots of Col. Wild-type plants were germinated on MS agar plate for 10 days and then shifted to MS (+Fe, control) and Fe-deficient (−Fe) for 7 days. ACTIN8 was used as the internal control. Values are means and SD from three to four independent biological replicates. Statistically significant differences (Tukey’s test, p < 0.05) are marked by different lowercase letters. C MNB1 protein level under Fe-deficient stress. 35S:MNB1-GFP transgenic seedlings germinated on MS agar plate for 10 days and then shifted to MS (+Fe, control) and Fe-deficient (−Fe) for 7 days. Protein extracts from treated seedlings and analyzed by 10% SDS-PAGE and Western blot assay. Anti-GFP antibody (upper panel), Rubisco (lower panel) as control. Original images of full-length gels or blots (Additional file 4: Fig. S4)