Fig. 2

Expression analysis of SiPKSA in sesame. A-B The expression profile of SiPKSA in different sesame tissues by RT-PCR (A) and qRT-PCR analysis (B). C-D RT-PCR (C) and qRT-PCR analysis (D) of the expression of SiPKSA in sesame fertile and sterile anthers at different developmental stages. S-t, S-md, and S-mp represent the tetrad stage, microspore development stage, and mature pollen stage of the sterile anthers, respectively. F-t, F-md, and F-mp represent the tetrad stage, microspore development stage, and mature pollen stage of the fertile anthers, respectively. Si-Actin (SIN_1009011) was used as the internal reference control. Three biological replicates were conducted and the error bars represent ± SD. Statistically significant differences were assessed using Student’s t-test (**p < 0.01 is considred a highly significantly different)