Table 5 Analysis of fatty acid composition of T1 and T2 seeds
From: Clone and Function Verification of the OPR gene in Brassica napus Related to Linoleic Acid Synthesis
Strain | Saturated fatty acids (%) | Unsaturated fatty acid (%) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
Palmitic acid | Stearic acid | Total | Oleic acid | Linoleic acid | Linolenic acid | Arachidonic acid | Erucic acid | Total | |||
T1 | Contrast T1 | 7.851 ± 0.37 | 2.947 ± 0.14 | 10.798 | 15.145 ± 3.51 | 28.204 ± 2.31 | 19.115 ± 1.53 | 19.925 ± 2.05 | 0 ± 0.06 | 82.389 | |
OPR1 | OPR1i | 6.8309 ± 0.277 | 3.3757 ± 0.221 | 10.2066 | 22.5651 ± 2.401 | 25.1403 ± 1.838 | 14.231 ± 1.057 | 18.7597 ± 1.975 | 1.2141 ± 0.535 | 81.9102 | |
Increase or decrease | -12.99%** | 14.55%** | -5.48%* | 48.99%** | -10.86%** | -25.55%** | -5.85%* | - | -0.58% | ||
OPR1-OE | 9.5163 ± 1.048 | 1.3951 ± 0.438 | 10.9114 | 20.5827 ± 1.609 | 32.2019 ± 1.666 | 19.79963 ± 7.508 | 18.3739 ± 3.331 | 0.2149 ± 0.257 | 91.17303 | ||
Increase or decrease | 21.21%** | -52.66%** | 1.05% | 35.90%** | 14.17%** | 3.58%* | -7.78%* | - | 10.66%** | ||
OPR2 | OPR2i | 7.2108 ± 0.384 | 3.2877 ± 0.183 | 10.4985 | 16.8825 ± 1.533 | 26.7364 ± 3.095 | 17.4696 ± 1.08 | 19.9638 ± 0.183 | 0.0473 ± 0.100 | 81.0996 | |
Increase or decrease | -8.15%* | 11.56%** | -2.77%* | 11.47%** | -5.20%* | -8.61%* | 0.19% | - | -1.57% | ||
OPR2-OE | 9.1207 ± 0.584 | 1.1043 ± 0.523 | 10.225 | 21.4321 ± 3.32 | 31.029 ± 1.838 | 17.0149 ± 1.747 | 18.1661 ± 2.313 | 0.3125 ± 0.323 | 87.9546 | ||
Increase or decrease | 16.17%** | -62.53%** | -5.31%* | 41.51%** | 10.02%* | -10.99%* | -8.83%* | - | 6.76%* | ||
OPR3 | OPR3i | 7.3383 ± 0.438 | 2.8731 ± 1.018 | 10.2114 | 18.318 ± 2.432 | 27.7582 ± 1.057 | 17.5874 ± 1.266 | 19.3568 ± 0.831 | 0.1795 ± 0.384 | 83.1999 | |
Increase or decrease | -6.53%* | -2.51% | -5.43% | 20.95%** | -1.58% | -7.99%* | -2.85% | - | 0.98% | ||
OPR3-OE | 10.2309 ± 1.924 | 1.351 ± 0.566 | 11.5819 | 22.9638 ± 1.382 | 32.2931 ± 3.819 | 18.9167 ± 2.314 | 14.2964 ± 1.938 | 0.2542 ± 0.252 | 88.7242 | ||
Increase or decrease | 30.31%** | -54.16%** | 7.26%* | 51.63%** | 14.50%** | -1.04% | -28.25%** | - | 7.69%* | ||
OPR4 | OPR4i | 7.3477 ± 0.29 | 3.0894 ± 0.253 | 10.4371 | 19.9233 ± 5.850 | 26.4398 ± 3.586 | 16.0365 ± 2.200 | 18.3035 ± 2.500 | 1.5821 ± 0.399 | 82.2852 | |
Increase or decrease | -6.41%* | 4.83%* | -3.34% | 31.55%** | -6.26%* | -16.11%** | -8.14%* | - | -0.13% | ||
OPR4-OE | 9.5807 ± 0.799 | 1.0081 ± 0.340 | 10.5888 | 20.9489 ± 3.030 | 31.4643 ± 0.984 | 19.8484 ± 7.047 | 15.3593 ± 1.484 | 0.2263 ± 0.282 | 87.8472 | ||
Increase or decrease | 22.03%** | -65.79%** | -1.94% | 38.32%** | 11.56%** | 3.84%* | -22.91%** | - | 6.62%* | ||
T2 | Contrast T2 | 9.09 ± 0.564 | 3.48 ± .0154 | 12.57 | 11.742 ± 2.154 | 27.486 ± 2.345 | 20.848 ± 1.253 | 18.165 ± 2.078 | 1.635 ± 0.178 | 79.876 | |
OPR1 | OPR1i | 8.463 ± 0.246 | 3.59 ± 0.784 | 12.053 | 9.23 ± 1.546 | 27.3638 ± 1.648 | 23.574 ± 5.791 | 18.205 ± 0.634 | 1.88 ± 0.481 | 80.2528 | |
Increase or decrease | -6.90%* | 3.16%* | -4.11%* | -21.39%** | -0.44% | 13.08%** | 0.22% | 14.98%** | 0.47% | ||
OPR1-OE | 10.217 ± 0.794 | 4.558 ± 0.254 | 14.775 | 9.897 ± 2.364 | 30.153 ± 0.994 | 21.122 ± 2.164 | 17.349 ± 1.424 | 1.645 ± 0.251 | 80.166 | ||
Increase or decrease | 12.40%* | 30.98%** | 17.54%** | -15.71%** | 9.70%* | 1.31% | -4.49%* | 0.61% | 0.36% | ||
OPR2 | OPR2i | 8.42 ± 0.284 | 4.048 ± 0.645 | 12.468 | 8.578 ± 3.045 | 27.71 ± 1.564 | 22.432 ± 1.578 | 17.839 ± 0.978 | 2.264 ± 4.165 | 78.823 | |
Increase or decrease | -7.37%* | 16.32%** | -0.81% | -26.95%** | 0.81% | 7.60%* | -1.79% | 38.47%** | -1.32% | ||
OPR2-OE | 10.039 ± 0.451 | 5.191 ± 0.214 | 15.23 | 9.44 ± 1.231 | 30.056 ± 0.597 | 21.467 ± 0.146 | 15.291 ± 0.548 | 1.91 ± 0.841 | 78.164 | ||
Increase or decrease | 10.44%* | 49.17%** | 21.16%** | -19.60%** | 9.35%* | 2.97% | -15.82%** | 16.82%** | -2.14% | ||
OPR3 | OPR3i | 8.856 ± 0.854 | 3.413 ± 0.847 | 12.269 | 8.259 ± 1.254 | 27.173 ± 0.914 | 24.266 ± 0.147 | 17.029 ± 0.934 | 2.244 ± 0.771 | 78.971 | |
Increase or decrease | -2.57% | -1.93% | -2.39% | -29.66%** | -1.14% | 16.39%** | -6.25%* | 37.25%** | -1.13% | ||
OPR3-OE | 9.433 ± 1.349 | 3.884 ± 0.915 | 13.317 ± 1.015 | 10.063 ± 0.187 | 28.715 ± 0.942 | 20.721 ± 0.975 | 16.84 ± 0.943 | 2.147 ± 0.143 | 78.486 | ||
Increase or decrease | 3.77% | 11.61%** | 5.94%* | -14.30%** | 4.47%* | -0.61% | -7.29%* | 31.31%** | -1.74% | ||
OPR4 | OPR4i | 9.133 ± 0.643 | 3.454 ± 0.916 | 12.587 ± 0.241 | 7.517 ± 1.214 | 27.208 ± 0.914 | 24.708 ± 0.511 | 17.791 ± 0.261 | 2.012 ± 0.646 | 79.236 | |
Increase or decrease | 0.47% | -0.75% | 0.14% | -35.98%** | -1.01% | 18.51%** | -2.06% | 23.06%** | -0.80% | ||
OPR4-OE | 8.757 ± 0.814 | 3.733 ± 0.646 | 12.49 | 11.382 ± 1.134 | 28.921 ± 0.615 | 20.511 ± 0.647 | 16.674 ± 1.057 | 1.611 ± 1.341 | 79.099 | ||
Increase or decrease | -3.66% | 7.27%* | -0.64% | -3.07% | 5.22%* | -1.62% | -8.21%* | -1.47% | -0.97% | ||