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Table 4 Primary and secondary structure analysis of each copy

From: Clone and Function Verification of the OPR gene in Brassica napus Related to Linoleic Acid Synthesis

structure

Analysis

OPR1

OPR2

OPR3

OPR4

Physical and chemical properties of primary structures

Number of amino acids

372

374

372

373

Molecular weight

41.4

41.57

41.32

41.28

Theoretical pI

5.55

6.01

5.88

6.22

Total number of positively charged residues(Arg + Lys)

46

44

43

42

Total number of negatively charged residues(Asp + Glu)

37

38

37

38

Formula

C1844H2838N508O550S15

C1844H2863N511O554S16

C1843H2839N509O546S15

C1835H2843N509O546S16

Instability index

38.82

35.76

39.65

38.57

Aliphatic index

75.27

75.64

75.27

75.6

Grand average of hydropathicity (GRAVY)

-0.355

-0.359

-0.343

-0.325

Secondary structure

Alpha helix (Hh)

31.18

31.55

31.18

30.56

Beta bridge (Bb)

0

0

0

0

Extended strand (Ee)

12.63

12.03

12.63

12.87

Beta turn (Tt)

6.72

6.42

7.26

8.04

Random coil (Cc)

49.46

50

48.92

48.53