| Â | A | T | C | G |
|---|
A | – | 4.23 | 6.67 | 11.75 |
T | 4.69 | – | 21.58 | 6.9 |
C | 4.69 | 13.7 | – | 6.9 |
G | 7.99 | 4.23 | 6.67 | – |
- Each entry shows the probability of substitution (r) from one base (row) to another base (column) [1]. For simplicity, the sum of r values is made equal to 100. Rates of different transitional substitutions are shown in bold and those of transversionsal substitutions are shown in italics. The nucleotide frequencies are 20.86% (A), 18.83% (T/U), 29.65% (C), and 30.67% (G). The transition/ transversion rate ratios are k1 = 1.704 (purines) and k2 = 3.238 (pyrimidines). The overall transition/ transversion bias is R = 1.16, where R = [A*G*k1 + T*C*k2]/[(A + G)*(T + C)]. This analysis involved 7 nucleotide sequences. Codon positions included were 1st + 2nd + 3rd + Noncoding. All ambiguous positions were removed for each sequence pair (pairwise deletion option). There were a total of 837 positions in the final dataset. Evolutionary analyses were conducted in MEGA X [2]
