Fig. 1
From: Chromatin-remodeling factor CHR721 with non-canonical PIP-box interacts with OsPCNA in Rice
Interaction analyses of OsPCNA and CHR721 (a) Yeast two-hybrid assay of OsPCNA and CHR721. The co-transformed strains were spotted on SD-Leu/-Trp/-His with 30 mM 3AT and SD-Ade/-Leu/-Trp/-His with 30 mM 3AT respectively. X-α-Gal was used for detecting GAL 4-based yeast two-hybrid interactions directly. 1,10–1 and 10–2 represented the decreasing quantities of co-transformed strains spotted on SD media. (b) LCI assay of OsPCNA and CHR721 in N. benthamiana leaves co-infiltrated with the agrobacterial strains containing OsPCNA-NLuc and CLuc-CHR721. The negative controls were marked in the image.