Fig. 7

Auxin-induced root organogenesis in the leaf explants is dependent on Ca²⁺ signals. A Effects of auxin on root organogenesis in EGTA-treated leaf explants. Leaf explants from the 9-day-old Col-0 seedlings were incubated on B5-agar plates containing 0.5 mM EGTA with or without 0.1 µM NAA. Biological triplicates were averaged and statistically analyzed using Student’s t-test (*, P < 0.05; difference from Mock). Whiskers indicate ± SD. Each replicate contains 24–30 explants. B-C Auxin responses in the leaf explants after EGTA treatment. Leaf explants from the 9-day-old DR5_rev:GFP transgenic seedlings were incubated on B5-agar plates containing 0.5 mM EGTA for the indicated time periods. GFP fluorescence and chlorophyll autofluorescence were analyzed using confocal microscopy (B). Scale bars indicate 0.2 mm. Relative intensity of GFP fluorescence near the wound site was measured using ImageJ software (C). Four biological replicates were averaged and statistically analyzed using Student’s t-test (*, P < 0.05; difference from Mock). Whiskers indicate ± SD