Fig. 2

ROS production is required for root organogenesis in the leaf explants. A-D Effects of DPI on ROS accumulation in the explants. Leaf explants from the 9-day-old Col-0 seedlings were incubated on B5-agar plates containing 1 µM DPI for the indicated time periods before NBT (A) or DAB (C) staining. DMSO was used for the Mock control. Relative intensity of NBT (B) and DAB (D) signals in the total leaf area and near the wound site was measured using ImageJ software. Seven to ten biological replicates were averaged and statistically analyzed using Student’s t-test (*, P < 0.05; difference from Mock). Scale bars indicate 0.5 mm. Whiskers indicate SD. E Effects of DPI on root organogenesis in the leaf explants. Leaf explants from the 9-day-old Col-0 seedlings were incubated on B5-agar plates containing 1 µM DPI up to 14 d. Rooting rates of three biological replicates were averaged and statistically analyzed using Student’s t-test (*, P < 0.05; difference from Mock). Each replicate contains 20–25 explants. Scale bars indicate 1 cm. Whiskers indicate ± SD