Fig. 8
From: Ethylene-induced potassium transporter AcKUP2 gene is involved in kiwifruit postharvest ripening
AcERF15 enhanced the activity of AcKUP2 promoter. A The growth status of yeasts on three different types of media (SD–Trp, SD–His–Leu–Trp, and SD–His–Leu–Trp + X–α–Gal) after the yeasts were transformed with a combination of effector and reporter vectors is shown. B The CDS of AcERFs were cloned into the pGreenII 0029 62–SK vector driven by the 35S promoter as an effector, and the promoter sequence of AcKUP2 was inserted into the pGreenII 0800–LUC vector as reporter. Dual-luciferase assays in Nicotiana benthamiana leaves were performed to analyse the activity enhancement of AcKUP2 promoter by AcERFs. An empty vector was used as the reference control. “**” indicates significant differences at P < 0.01