Table 7 Primer sequences of the selected GSL side-chain modification genes in I. indigotica
From: Insights into glucosinolate accumulation and metabolic pathways in Isatis indigotica Fort.
Primer Name | Forward Primer (5′-3′) | Reverse Primer (5′-3′) | Length of Products (bp) | Related Gene Name |
|---|---|---|---|---|
eIF2 | TACCAGTGGCTCGCTTGAC | CAACCAAAGCAAATGACGTACTC | 117 | eIF2 |
PP2A-4 | GAATGCCTGCGAAAGTATGG | TCCTAATGTTGTCAAGGGTCTC | 151 | PP2A-4 |
18849–1 | CATATTCCATAACCCGCAGG | GTTACTGTAATACTTCACCAGCTGT | 312 | GSL-OH-1 |
18849–2 | CCTAAACCTTCCTCGGTGGT | CGTCTTTAAGCTCCGCAACA | 106 | GSL-OH-2 |
7910 | GCTGGTGAAGTATAGCAGTAACTC | CGCAACCCTAAAGCTTCTGAT | 207 | GSL-OH-3 |
13981 | ATTACTACCCGCCTTGTCCA | CGGTTCTTCGCCTCTGTTC | 228 | GSL-OH-like-1 |
Tri14631 | GGGCTCTTCTCGTCAACCT | GCGTGTACTCAGTGACGGTAG | 251 | GSL-OH-like-2 |