Fig. 4

Hyperosmotic stress transiently depolymerizes interphase microtubules in a PHS1-dependent manner. (a) Immunohistochemical images of microtubules (green) and acetylated microtubules (red), which mainly represented flagella microtubules, after application of 0.3 M sorbitol in wild type (WT), phs1 mutant, and a complimented line (comp. line). Bar: 10 μm. (b) Quantification of microtubule polymer amounts. ***; statistically significant difference at p < 0.01 by one-way ANOVA with Tukey-HSD post-hoc test, when compared with wild-type values. Error bars indicate standard deviations from ten cells for each genotype