Fig. 5

Validation of RNA-Seq results by qRT-PCR using 14 L. bicolor candidate genes. The expression levels of 14 randomly selected genes under different treatment are shown. A red color indicates that the gene is highly expressed under the corresponding treatment. CK (0 mM NaCl), plants cultivated with only Hoagland nutrient solution; 0+5 (0 mM NaCl + 5 µM MT), plants cultivated with Hoagland nutrient solution plus 5 μM melatonin; 300 (300 mM NaCl), plants cultivated with Hoagland nutrient solution plus 300 mM NaCl; 300+5 (300 mM NaCl + 5 µM MT), plants cultivated with Hoagland nutrient solution plus 300 mM NaCl and 5 μM melatonin