Fig. 5

CRISPR/Cas9-mediated mutation of the SlSRM1-like gene. A Schematic diagram of the sgRNA target sites on the SlSRM1-like gene. The exons are indicated by yellow boxes, and introns and intergenic sequences are indicated by straight lines. The arrow indicates the direction of the genome, from 5′ to 3′. The black dots in the yellow box represent the sgRNA target sites whose sequence is shown next to them. The PAM motifs are marked in red. Bar = 100 bp. B Sequencing results of the sequence near the mutation site of the SlSRM1-like gene. The lower part of the sgRNA target site is marked with a red line, and the PAM motif is enclosed in a black box. C Amino acid sequence alignment of SlSRM1-like and SlSRM1-like-Ms proteins. DNAMAN 6.0 software was used for sequence alignment