Fig. 2

Tracing auxin-responsive reporters in tissues of transgenic Arabidopsis thaliana lines grown on NO₃⁻ (control) or NH₄⁺ as the only nitrogen source. A Confocal images of DR5::GFP expression. Overlay of transmission light and green fluorescence and detached green channel on the right respectively. Scale bars for leaves represent 100 μm and for roots 50 μm. B Photographs of DR5::GUS staining in tissues. For whole-leaf pictures scale bars represent 1 mm and for higher magnification 100 μm. Photos for root tips and lateral roots (from the differentiation zone) with developing higher-order lateral root primordia were selected. In roots scale bars for lower magnification represent 100 μm, for higher magnification 10 μm. Images shown are representative of at least five independent replicates shown in the Supplement (Supplemental Figs. 1, 2 and 3)