Fig. 3

Confirmation of the expression patterns of mRNAs (A) and lncRNAs (B) using qPCR. Ten mRNAs and ten lncRNAs were randomly selected for expression analysis during the fiber developmental stages of G. hirsutum ND13 treated with NI and WD. The correlation of relative expression for mRNAs and lncRNAs measured by RNA-seq and qPCR was estimated with r-values. UBQ7 was used as the reference gene. Gene (mRNA) IDs are shown in the genome of G. hirsutum TM-1 (NAU-NBI v1.1), including Gh_A05G0770 (17.3 kDa class I heat shock protein), Gh_A09G1977 (1-aminocyclopropane-1-carboxylate oxidase homolog 1), Gh_A11G2903 (ABC transporter G family member 2), Gh_D01G0047 (Protein RADIALIS-like 6), Gh_D03G1452 (Tubulin beta-7 chain), Gh_D04G0942 (No annotation), Gh_D05G1621 (No annotation), Gh_D08G1970 (Probable aquaporin PIP1-2), Gh_D08G2730 (Bidirectional sugar transporter SWEET15), and Gh_Sca115726G01 (Aspartic proteinase nepenthesin 1)