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Fig. 4 | BMC Plant Biology

Fig. 4

From: Molecular cytogenetics for a wheat–Aegilops geniculata 3Mg alien addition line with resistance to stripe rust and powdery mildew

Fig. 4

Karyotypes with the genomic composition variation of W19513, which was obtained by using FISH and sequential FISH-GISH analysis. FISH used Oligo-pSc119.2 (green) and Oligo-pTa535 (red) as probes. The SY159 genomic DNA was used as probe for sequential FISH- GISH. The red arrow indicates the introduction of alien chromosomes of SY159. White arrows indicated the structural variation of the chromosomes 4A and 6B. A FISH of W19513. B GISH of W19513 in the same cell. C Analysis of CS by FISH. D FISH signal comparison between CS and W19513 on chromosomes 4A, 6B. Chromosomes were counterstained by DAPI (blue). Bar indicates 10 μm

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BMC Plant Biology

ISSN: 1471-2229

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