Fig. 2

The mean of MT fluorescence intensity and categories allow expressing the degree of MT polymerization in microspores. A The mean IOD at 488 nm excitation [a.u.] of MT in fresh microspores (control; FM), exposed to the 0.7 M mannitol stress treatment (MAN) or mannitol and n-butanol treatment (MANB), and during MAN or MANB stress recovering in MS3M medium at 4 or 8 hours (MAN-R4, MAN-R8 and MANB-R4, respectively). Analysis of variance was carried out using GLM procedure of SAS. The Duncan method (α≤0.05) was used for mean separation. Treatments followed by the same letter are not significantly different (P≤0.05). B Distribution of IOD categories [a.u.] as a function of the polymerization of MT in FM microspores, after MAN, MAN-R4, MAN-R8, MANB, and MANB-R4