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Fig. 7 | BMC Plant Biology

Fig. 7

From: Long terminal repeats (LTR) and transcription factors regulate PHRE1 and PHRE2 activity in Moso bamboo under heat stress

Fig. 7

The yeast one-hybrid assay showing TCP20, DOF2, and GATA transcription factors (TFs) interactions with PHRE1 and PHRE2 LTR. The bait constructs pLacZi -PHRE1, pLacZi -PHRE2, and prey constructs pGADT7- TCP20/DOF2, pGADT7- GATA respectively were co-transformed into yeast strain EGY48, and the interactions were examined on SD/-Ura/-Trp/X-Gal plates. The blue color on the plate indicates the TCP20, DOF2, and GATA TF's interaction with LTRs. Plasmid pairs pGADT7-Tag + pLacZi - P53 acted as positive and negative controls. Each yeast colony was dissolved in 100μl of sterilized water and diluted 10–1 to 10–3. At least three colonies per combination were tested and analyzed

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BMC Plant Biology

ISSN: 1471-2229

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