Fig. 1

Phylogenetic analysis of INV proteins between different species. Phylogenetic tree was constructed with INV proteins of Arabidopsis thaliana (blue), Oryza sativa (purple), Zea may (green), Vitis vinifera (red), Malus domestica (cyno), and P. trifoliata (black). Multiple sequences alignment of INV proteins was conducted by MEGAX with the ClustalW method. The tree was constructed with the neighbor-joining method and bootstraps with 1000 replicates for poisson correction model. The dendrogram was categorized into five distinct subfamilies that highlighted using different colors. The light brown color refers to the cytoplasm localized INV proteins. The pink and purple colored clusters represent the chloroplast and mitochondria targeted INV isoforms, respectively. The acidic INV group was divided into cell wall bound INV peptides with light green color and vacuolar INV proteins with dark green color. The scale bar indicates to the relative amount of change along the branches. Accession number can be found in Supplementary Table S2