Fig. 4
pTRV-RNA1 and pTRV-RNA2 VIGS vectors based on the TRV California isolate. A pTRV-RNA1 carrying the full length TRV California RNA1 interrupted by an intron on its T-DNA. B pTRV-RNA2 carrying the coat protein and the 3′ and 5′ regions from TRV California RNA2. The gray sections of the circles represent the T-DNA, and the black sections represent the vector backbone originating from the binary plant transformation vector pSOL9DEF1. Abbreviations: i, intron 3 of the A. thaliana NIA1 gene for nitrate reductase (Z19050); LB, left border; ntpII, neomycin phosphotransferase II; ori ColE1, origin of replication of ColE1; ori pVS1, origin of replication of pVS1; P35S, 35S promoter from cauliflower mosaic virus; RB, right border; RdR, RNA-dependent RNA polymerase; repA pVS1, replication protein from pVS1; staA pVS1, plasmid stability protein from pVS1; T35S, 35S terminator from cauliflower mosaic virus