Table 1 Summary of individual SNP chip identification in F1 generation
Sample number | With or without wax | Value of Fluorescence flow cytometry | Rate of homozygous site (%) | Homozygous and different site number of parents | Number of sites same as the female parent | Male parent infiltration rate (%) | Hybridization site rate (%) |
|---|---|---|---|---|---|---|---|
Z1–1 | No | 380,167.50 | 93.43 | 6377 | 6361 | 0.00 | 0.00 |
Z1–2 | No | 402,510.90 | 93.29 | 6377 | 6364 | 0.00 | 0.00 |
Z1–3 | No | 357,577.39 | 93.31 | 6377 | 6370 | 0.00 | 0.00 |
Z1–4 | No | 393,843.57 | 93.18 | 6377 | 6359 | 0.00 | 0.03 |
Z1–5 | No | 379,213.62 | 93.25 | 6377 | 6368 | 0.00 | 0.00 |
Z1–6 | No | 370,379.61 | 93.35 | 6377 | 6360 | 0.00 | 0.02 |
Z1–7 | No | 396,666.50 | 93.52 | 6377 | 6360 | 0.00 | 0.00 |
Z2–1 | No | 379,647.85 | 93.71 | 6569 | 6551 | 0.20 | 0.00 |
Z2–2 | No | 403,773.06 | 93.65 | 6569 | 6550 | 0.20 | 0.00 |
Z2–3 | No | 365,186.36 | 92.93 | 6569 | 6499 | 0.00 | 0.06 |
Z3–1 | No | 369,954.25 | 93.66 | 6660 | 6649 | 0.00 | 0.00 |
Z3–2 | No | 373,199.38 | 93.64 | 6660 | 6649 | 0.00 | 0.00 |
Z3–3 | No | 370,213.29 | 93.61 | 6660 | 6649 | 0.00 | 0.00 |
Z4–1 | Yes | 380,411.61 | 59.71 | 8147 | 356 | 0.64 | 92.91 |
Z4–2 | No | 358,807.06 | 93.23 | 8147 | 8120 | 0.00 | 0.01 |
Z4–3 | No | 366,565.08 | 93.35 | 8147 | 8135 | 0.00 | 0.00 |
Z5–1 | Yes | 356,672.96 | 62.73 | 9233 | 324 | 3.58 | 90.40 |
Z5–2 | No | 370,918.46 | 93.37 | 9233 | 9224 | 0.00 | 0.00 |
Z5–3 | No | 397,723.89 | 93.60 | 9233 | 9218 | 0.00 | 0.01 |
Z5–4 | Yes | 403,230.98 | 60.77 | 9233 | 64 | 0.41 | 96.64 |
Z5–5 | No | 384,689.82 | 93.63 | 9233 | 9220 | 0.00 | 0.00 |
Z6–1 | No | 375,084.27 | 93.61 | 9130 | 9101 | 0.00 | 0.16 |
Z6–2 | No | 390,668.40 | 93.23 | 9130 | 9087 | 0.01 | 0.07 |
Z6–3 | No | 384,049.43 | 93.49 | 9130 | 9095 | 0.24 | 0.00 |
Z7–1 | No | 454,023.50 | 92.72 | 10,469 | 10,409 | 0.00 | 0.43 |
Z8–1 | No | 385,795.47 | 93.66 | 6730 | 6719 | 0.09 | 0.00 |
Z8–2 | No | 365,029.51 | 93.60 | 6730 | 6721 | 0.09 | 0.00 |
Z9–1 | Yes | 578,710.61 | 64.61 | 7236 | 3008 | 0.11 | 46.41 |
Z9–2 | Yes | 369,157.11 | 66.73 | 7236 | 3070 | 4.99 | 50.72 |
Z10–1 | No | 359,277.98 | 98.70 | 9338 | 9308 | 0.01 | 0.09 |
Z10–2 | Yes | 365,940.57 | 60.45 | 9338 | 56 | 0.51 | 97.34 |
Z10–3 | Yes | 319,310.70 | 60.76 | 9338 | 52 | 0.82 | 96.84 |
Z11–1 | No | 375,536.34 | 93.54 | 6972 | 6917 | 0.65 | 0.01 |
Z11–2 | No | 333,786.55 | 93.52 | 6972 | 6920 | 0.65 | 0.01 |
Z11–3 | No | 345,056.30 | 93.52 | 6972 | 6919 | 0.65 | 0.01 |
Z12–1 | No | 385,472.76 | 93.53 | 8364 | 8315 | 0.30 | 0.19 |
Z12–2 | Yes | 315,531.95 | 63.54 | 8364 | 75 | 0.84 | 96.32 |
Z12–3 | No | 368,941.21 | 93.62 | 8364 | 8326 | 0.29 | 0.00 |
Z13–1 | No | 379,745.28 | 93.07 | 7731 | 7650 | 0.52 | 0.26 |
Z13–2 | No | 373,948.74 | 93.29 | 7731 | 7687 | 0.14 | 0.27 |
Z13–3 | No | 389,945.59 | 92.93 | 7731 | 7664 | 0.04 | 0.72 |
Z14–1 | Yes | 362,004.55 | 62.72 | 8801 | 50 | 1.59 | 96.17 |
Z14–2 | Yes | 334,489.64 | 62.15 | 8801 | 54 | 1.00 | 96.74 |
Z14–3 | Yes | 396,302.44 | 62.23 | 8801 | 58 | 0.97 | 95.40 |
Z15–1 | Yes | 494,644.08 | 67.01 | 8163 | 50 | 10.62 | 86.49 |
Z15–2 | Yes | 442,013.75 | 66.64 | 8163 | 3846 | 4.47 | 46.00 |
Z15–3 | Yes | 394,262.46 | 64.85 | 8163 | 3983 | 0.28 | 49.04 |
Z16–1 | Yes | 355,685.45 | 64.57 | 7606 | 102 | 0.75 | 95.24 |
Z16–2 | Yes | 299,415.21 | 64.16 | 7606 | 102 | 0.76 | 95.15 |
Z17–1 | Yes | 464,444.93 | 62.49 | 8373 | 50 | 0.74 | 94.88 |
Z17–2 | No | 439,435.82 | 93.52 | 8373 | 8330 | 0.26 | 0.01 |
Z17–3 | No | 412,492.42 | 93.51 | 8373 | 8333 | 0.26 | 0.01 |
Z18–1 | No | 365,742.23 | 93.40 | 6156 | 6105 | 0.47 | 0.15 |
Z18–2 | No | 374,924.44 | 93.16 | 6156 | 6105 | 0.47 | 0.18 |
Z18–3 | No | 352,318.10 | 93.15 | 6156 | 6090 | 0.47 | 0.41 |
Z18–4 | No | 398,995.37 | 93.19 | 6156 | 6086 | 0.83 | 0.08 |
Z19–1 | No | 650,382.52 | 62.40 | 7881 | 3900 | 0.38 | 48.67 |
Z19–2 | No | 643,543.85 | 93.20 | 7881 | 7835 | 0.03 | 0.11 |
Z19–3 | No | 600,221.32 | 93.20 | 7881 | 7817 | 0.05 | 0.33 |
Z19–4 | No | 590,485.00 | 93.20 | 7881 | 7810 | 0.37 | 0.14 |
Z20–1 | No | 660,128.07 | 93.40 | 7683 | 7611 | 0.49 | 0.00 |
Z20–2 | No | 661,356.33 | 93.20 | 7683 | 7606 | 0.49 | 0.00 |
Z20–3 | No | 596,629.91 | 93.30 | 7683 | 7609 | 0.46 | 0.03 |
Z20–4 | No | 596,905.70 | 93.10 | 7683 | 7607 | 0.46 | 0.03 |