Fig. 6

Organ-specific analysis of the levels of the enzyme-coding genes and the metabolites within the biosynthetic pathway of indole diterpene alkaloids in Bletilla striata. A The biosynthetic pathway of indole diterpene alkaloids referring to the KEGG database (https://www.kegg.jp/kegg-bin/show_pathway?map00403) [13]. Based on the transcriptome assembly annotated by the KEGG database, two types of enzymes could find their coding genes (colored within the pathway) based on the EC numbers. PaxC: geranylgeranyldiphosphate transferase; PaxB: paspaline synthase; PaxD: dimethylallyldiphosphate transferase; LtmE: dimethylallyldiphosphate transferase; PaxM: FAD dependent monooxygenase, [EC:1.14.13.-]; PaxP: cytochrome P450 monooxygenase, [EC:1.14.13.-]; PaxQ: cytochrome P450 monooxygenase, [EC:1.14.13.-]; AtmQ: cytochrome P450 monooxygenase, [EC:1.14.13.-]; TerQ: cytochrome P450 monooxygenase, [EC:1.14.13.-]; TerP: cytochrome P450 monooxygenase, [EC:1.14.13.-]; LtmQ: cytochrome P450 monooxygenase, [EC:1.14.13.-]; LtmP: cytochrome P450 monooxygenase, [EC:1.14.13.-]; LtmJ: cytochrome P450 monooxygenase, [EC:1.14.13.-]; AtmD: dimethylallyldiphosphate transferase, [EC:2.5.1.-]; LtmF: dimethylallyldiphosphate transferase, [EC:2.5.1.-]; LtmK: cytochrome P450 monooxygenase, [EC:1.14.21.-]. B A heatmap showing the expression patterns of the enzyme-coding genes in roots, tubers and leaves of B. striata. After z-score normalization, the heatmap was drawn at a log2 scale from -3 to +2. C A heatmap showing the levels of the metabolites in three organs. Based on metabolite quantification, the heatmaps were drawn at a log2 scale from -4 to +2. Terpendole B: C20528; beta-PC-M6: C20535; Paspalicine: C20553; Aflatrem: C20555; 10,11-Epoxy-3-geranylgeranylindole: C20526; Emindole SB: C20527; Terpendole G: C20586; Geranylgeranyl diphosphate: C00353; 20,21-Diprenylterpendole J: C20594; Indoleglycerol phosphate: C03506; 13-Desoxypaxilline: C20531; 14,15-Epoxyemindole SB: C20529