Fig. 5

Organ-specific analysis of the levels of the enzyme-coding genes and the metabolites within the biosynthetic pathway of volatile terpenoids in Bletilla striata. A The previously reported MVA biosynthetic pathway of volatile terpenoids [25]. Based on the transcriptome assembly annotated by the KEGG database, eight enzymes could find their coding genes (colored within the pathway) based on the EC numbers. B The previously reported MEP biosynthetic pathway of volatile terpenoids [33]. Based on the transcriptome assembly annotated by the KEGG database, seven enzymes could find their coding genes (colored within the pathway) based on the EC numbers. AACT: acetoacetyl-CoA thiolase, [EC:2.3.1.9]; HMGS: HMG-CoA synthase, [EC:2.3.3.10]; HMGR: HMG-CoA reductase, [EC:1.1.1.34]; MVK: mevalonate kinase, [EC:2.7.1.36]; PMK: phosphomevalonate kinase, [EC:2.7.4.2]; MVD: mevalonate diphosphate decarboxylase, [EC:4.1.1.33]; IDI: isopentenyl diphosphate isomerase, [EC:5.3.3.2]; GDS: geranyl diphosphate synthase, [EC:2.5.1.1]; FDS: farnesyl diphosphate synthase, [EC:2.5.1.10], [EC:2.5.1.68], [EC:2.5.1.92]; TPS: terpene synthase, [EC:4.2.3.47], [EC:4.2.3.49]; DXS: 1-deoxy-D-xylulose-5-phosphate synthase, [EC:2.2.1.7]; DXR: 1-deoxy-D-xylulose-5-phosphate reductoisomerase, [EC:1.1.1.267]; MCT: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, [EC:2.7.7.60]; CMK: CDP-ME kinase, [EC:2.7.1.148]; MDS: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, [EC:4.6.1.12]; HDS: (E)-4-hydroxy-3-methylbut-2-enyl diphosphate synthase, [EC:1.17.7.1], [EC:1.17.7.3]; HDR: (E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase, [EC:1.17.7.4]; GGDS: geranyl geranyl diphosphate synthase, [EC:2.5.1.29]. C A heatmap showing the expression patterns of the enzyme-coding genes, belonging to the MVA pathway, in roots, tubers and leaves of B. striata. In most cases, there are two or more coding genes. After z-score normalization, the heatmap was drawn at a log2 scale from -2 to +3. D A heatmap showing the expression patterns of the enzyme-coding genes, belonging to the MEP pathway, in three organs of B. striata. For some of the enzymes, there are two or more coding genes. After z-score normalization, the heatmap was drawn at a log2 scale from -2 to +3. E A heatmap showing the levels of the metabolites in three organs. Based on metabolite quantification, the heatmaps were drawn at a log2 scale from -4 to +3. Farnesyl diphosphate: C00448, C16826, C19760; MEP: 2-C-methyl-D-erythritol 4-phosphate, C11434; MVA: mevalonate, C00418; I diphosphate: Isopentenyl diphosphate, C00129, C00235; D diphosphate: Dimethylallyl diphosphate, C00235; MVA-5-phosphate: C01107; Geranyl geranyl diphosphate: C00353