Fig. 4

Organ-specific analysis of the levels of the enzyme-coding genes and the metabolites within the flavonoid biosynthetic pathway of Bletilla striata. A The previously reported biosynthetic pathway of flavonoids [12]. Based on the transcriptome assembly annotated by the KEGG database, six enzymes could find their coding genes (colored within the pathway) based on the EC numbers. CHS: chalcone synthase, [EC:2.3.1.74]; CHI: chalcone isomerase, [EC:5.5.1.6]; FLS: flavonol synthase, [EC:1.14.20.6]; DFR: dihydroflavonol-4-reductase, [EC:1.1.1.219]; LAR: leucoanthocyanidin reductase, [EC:1.17.1.3]; ANR: anthocyanidin reductase, [EC:1.3.1.77]; ANS: anthocyanidin synthase, [EC:1.14.20.4]; F3H: flavanone 3-hydroxylase, [EC:1.14.11.9]; F3’H: flavonoid 3’-hydroxylase, [EC:1.14.14.82]; IFS: isoflavone synthase. B A heatmap showing the expression patterns of the enzyme-coding genes in roots, tubers and leaves of B. striata. In most cases, there are two or more coding genes. After z-score normalization, the heatmap was drawn at a log2 scale from -3 to +3. C A heatmap showing the levels of the metabolites in three organs. Based on metabolite quantification, the heatmaps were drawn at a log2 scale from -2 to +2. Flavanone: C00766