Fig. 2

Organ-specific analysis of the levels of the enzyme-coding genes and the metabolites within the biosynthetic pathway of Bletilla striata polysaccharide (BSP). A The previously reported biosynthetic pathway of BSP [10] was employed for this analysis. Based on the transcriptome assembly annotated by the KEGG database, nine enzymes could find their coding genes (colored within the pathway) based on the EC numbers. scrA: phosphotransferase system, [EC:2.7.1.211]; sacA: beta-fructofuranosidase, [EC:3.2.1.26]; HK: hexokinase, [EC:2.7.1.1]; scrK: fructokinase, [EC:2.7.1.4]; GPI: glucose-6-phosphate isomerase, [EC:5.3.1.9]; pgm: phosphoglucomutase, [EC:5.4.2.2]; UGP2: uridine-diphosphate glucose pyrophosphorylase, [EC:2.7.7.9]; manA: mannose-6-phosphate isomerase, [EC:5.3.1.8]; PMM: phosphomannomutase, [EC:5.4.2.8]; GMPP: mannose-1-phosphate guanylyltransferase, [EC:2.7.7.13]. B A heatmap showing the expression patterns of the enzyme-coding genes in roots, tubers and leaves of B. striata. In most cases, there are two or more coding genes for each enzyme. After z-score normalization, the heatmap was drawn at a log2 scale from -2 to +3. C A heatmap showing the levels of the metabolites in three organs. Based on metabolite quantification, the heatmaps were drawn at a log2 scale from -2 to +2. Suc: sucrose, C00089; Fru: fructose, C01496, C02336; Man: mannose, C00159