Fig. 1

Organ-specific metabolic profiles of Bletilla striata. A Leaves, tubers and roots of the two-year old seedlings of B. striata were collected for untargeted metabolic profiling and transcriptome sequencing. B Principal component analysis (PCA) indicates that the metabolic profile obtained under the positive ion mode has an organ-specific feature. C PCA indicates that the metabolic profile obtained under the negative ion mode also has an organ-specific feature. D A heatmap showing the levels of the metabolites identified from the three organs of B. striata under the positive ion detection mode of ultra-performance liquid chromatography—tandem mass spectrometry (UPLC-MS/MS). E A heatmap showing the levels of the metabolites identified from the three organs of B. striata under the negative ion detection mode of UPLC-MS/MS. Based on metabolite quantification, the heatmaps were drawn at a log2 scale from -4 to +4. F Histogram showing the top 20 KEGG pathways, to which the MS1-level metabolites identified under the positive ion mode were annotated. G Histogram showing the top 20 KEGG pathways, to which the MS1-level metabolites identified under the negative ion mode were annotated. H KEGG-based annotations of the transcriptome assembly